| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phelps, Anna E (9)
|
MAUN-0 |
34.05 |
34.63 |
0.58 |
| 2 |
Zerfass, Ava D (9)
|
MAEMAC |
42.94 |
40.20 |
-2.74 |
| 3 |
Collins, Ryleigh (9)
|
MAPFY |
43.30 |
41.22 |
-2.08 |
| 4 |
Iacobelli, Sophia C (9)
|
MAEMAC |
39.79 |
41.71 |
1.92 |
| 5 |
Tassinari, Marianne E (9)
|
MAPFY |
|
41.78 |
- |
| 6 |
Lenett, Carly A (9)
|
MAEMAC |
|
45.43 |
- |
| 7 |
Frank, Alice C (9)
|
MAEMAC |
48.61 |
45.80 |
-2.81 |
| 8 |
Karnhaukhova, Anastasiya (9)
|
MAPJA |
58.08 |
47.92 |
-10.16 |
| 9 |
Lin, Simone (9)
|
MAPAAC |
53.91 |
49.55 |
-4.36 |
| 10 |
Piacenti, Lily (9)
|
MAPFY |
|
50.52 |
- |
| 11 |
Settle, Ashley (9)
|
MAUNPA |
|
54.57 |
- |
| 12 |
Wiragh, Kendall J (9)
|
MAEMAC |
|
58.71 |
- |
|
Sadler, Cheyenne N (9)
|
MAUNAA |
1:07.84 |
DQ |
- |